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For research use only. The Rox FIX-A kit or quantitative determination of human Factor IXa (FIXa) contamination in human Factor IX (FIX) concentrates. FIXa activity in FIX concentrates is determined in a chromogenic method, in which human FX is activated by contaminating human FIXa in the FIX concentrate in the presence of FVIII, thrombin, calcium ions and phospholipid. The amount of generated human FXa is determined from the hydrolysis of a chromogenic FXa substrate. The sample FIXa activity is determined by the slope ratio model in which the potency of the sample is calculated vs. a FIXa standard with potency expressed in International Units (IU).